James Stewart Jr.
Office: 220 Harned Hall
Phone: (662) 325-4683
Bachelor of Science Microbiology; College of Sciences and Mathematics, Auburn University, Auburn, Alabama
Ph.D. Biomedical Sciences, College of Veterinary Medicine, Auburn University, Auburn, Alabama
Post Doctoral Fellowships
Department of Developmental Biology and Anatomy, University South Carolina School of Medicine, Columbia, South Carolina
Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, New Orleans, Louisiana
Primary Investigator, Center for Cardiovascular and Pulmonary Research, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio
Research Assistant Professor, Department of Pediatrics, The Ohio State University, Columbus, Ohio
The current focus of my laboratory is to investigate the role of cardiac fibroblasts in type 2 diabetes mellitus‐induced cardiomyopathic conditions. Using knockout mice with a targeted gene deletion for the leptin receptor (db/db) mice, alterations in basal activation of signaling cascades and expression of pro‐fibrotic markers within the myocardium of diabetic (db/db) mice compared to non‐diabetic (Db/db) littermates are being examined. In addition, experiments have been undertaken to examine the effects of increased mechanical load, increased oxidative stress, and anti‐oxidant therapy on fibroblasts in diabetic cardiomyopathy. Another focus of the Stewart laboratory is the role of the extracellular matrix (ECM) in diabetic remodeling. By utilizing three dimensional matrix technologies, we can simulate in vivo ECM‐cell interactions in an in vitro environment.
Selected Peer-reviewed Publications
Stewart, Jr., J.A., Wei, C.C., Brower, G.L., Hankes, G.H., Dillon, A.R., Rynders, P.E., Lucchesi, P.A., Janicki, J.S., and Dell'Italia, L.J. Cardiac mast cell‐ and chymasemediated matrix metalloproteinase activity and left ventricular remodeling in mitral regurgitation in the dog. J Mol Cell Cardiol 35, 311‐319, 2003.
Janicki, J.S., Brower, G.L., Gardner, J.D., Chancey, A.L., and Stewart, Jr., J.A. The dynamic interaction between matrix metalloproteinase activity and adverse myocardial remodeling. Heart Failure Reviews. 9, 33‐42, 2004.
Borg T.K., Stewart, Jr., J.A., and Sutton, M.A. Imaging the cardiovascular system: Seeing is believing. Microsc Microanal. 11, 189‐99, 2005.
Janicki, J.S., Brower, G.L., Gardner, J.D., Forman, M.F., Stewart, Jr., J.A., Murray, D.B., Chancey, A.L.Cardiac mast cell regulation of matrix metalloprotease‐related ventricular remodeling in chronic pressure or volume overload. Cardiovascular Research. 69: 657‐65, 2006.
Stewart, Jr., J.A., Cashatt, D.O., Borck , A.C., Brown, J.E., and Carver, W.E. 17β‐Estradiol modulation of angiotensin II‐stimulated response in cardiac fibroblasts. J Mol Cell Cardiol 41: 97‐107, 2006.
Zhang, X., Stewart, Jr., J.A., Kane, I.D., Massey, E.P., Cashatt, D.O., and Carver, W.E. Effects of elevated glucose on the interactions of cardiac fibroblasts with the extracellular matrix. In Vitro Cell Dev Biol Anim. 2007 Sep‐Oct;43(8‐9):297‐305. (Co‐First Author)
Stewart, Jr. J.A., Lazartigues, E. and Lucchesi, P.A. The angiotensin converting enzyme 2/Ang‐(1‐7) axis in the heart: a role for MAS communication? Circ Res. 2008 Nov 21;103(11):1197‐9.
Chicoine, L.G., Stewart, Jr., J.A., Lucchesi, P.A. Is resveratrol the magic bullet for pulmonary hypertension? Hypertension. 54:473‐4 2009.
Stewart, Jr., J.A., Massey, E.P., Fix, C., Zhu, J., Goldsmith, E.C., Carver, W. Temporal alterations in cardiac fibroblast function following induction of pressure overload. Cell Tissue Res. 340:117‐26. 2010.
Hutchinson, K.R., Stewart, Jr., J.A., Lucchesi, P.A. Extracellular matrix remodeling during the progression of volume overload‐induced heart failure. J Mol Cell Cardiol 48:564‐9. 2010.
Dodd, T., Jadhav, R., Wiggins, L., Stewart, J., Smith, E., and Rocic P. p38 MAPK-dependent MMP 2 and 9 regulation during coronary collateral growth. (Accepted Am. J. Physiology 2011).
Stewart, Jr., J.A., West, T.A. and Lucchesi, P.A. Nitric Oxide-induced Collagen IV Expression and Angiogenesis: FAK or Fiction? (Accepted- Am. J. Physiology 2011).
Paige Shattuck Katz, Ph.D.;Aaron J Trask, Ph.D.;Flavia M Souza-Smith, Ph.D.;Kirk R. Hutchinson, Ph.D.;Maarten Galantowicz;Kevin C Lord, Ph.D.;James A Stewart, Jr., Ph.D.;Mary J. Cismowski, Ph.D.;Kurt J Varner, Ph.D.;Pamela A Lucchesi, Ph.D. Coronary Arterioles in Type 2 Diabetic (db/db) Mice Undergo a Distinct Pattern of Remodeling Associated with Decreased Vessel Stiffness Basic Research in Cardiology 2011
Flavia M. Souza-Smith; Paige S. Katz; Aaron J. Trask.; James A Stewart, Jr.; Kevin C. Lord; Kurt J. Varner; Dalton V. Vassallo; Pamela A. Lucchesi. Mesenteric Resistance Arteries in Type 2 Diabetic db/db Mice Undergo Outward Remodeling. (Accepted PLoS ONE 2011).